Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins

作者: H.-X. Liu , M. Zhang , A. R. Krainer

DOI: 10.1101/GAD.12.13.1998

关键词: BiologyExonic splicing enhancerGeneticsExonExonic splicing silencerIntronRNA-binding proteinRNA splicingConsensus sequenceSR protein

摘要: … spliced in S100 extract plus any of the three SR proteins we examined. However, addition of two SR proteins did not increase its splicing … winners tested failed to splice in S100 extract …

参考文章(64)
G. R. Screaton, J. F. Cáceres, A. Mayeda, M. V. Bell, M. Plebanski, D. G. Jackson, J. I. Bell, A. R. Krainer, Identification and characterization of three members of the human SR family of pre‐mRNA splicing factors. The EMBO Journal. ,vol. 14, pp. 4336- 4349 ,(1995) , 10.1002/J.1460-2075.1995.TB00108.X
Y. Cavaloc, M. Popielarz, J.P. Fuchs, R. Gattoni, J. Stévenin, Characterization and cloning of the human splicing factor 9G8: a novel 35 kDa factor of the serine/arginine protein family. The EMBO Journal. ,vol. 13, pp. 2639- 2649 ,(1994) , 10.1002/J.1460-2075.1994.TB06554.X
I.C. Eperon, D.C. Ireland, R.A. Smith, A. Mayeda, A.R. Krainer, Pathways for selection of 5' splice sites by U1 snRNPs and SF2/ASF The EMBO Journal. ,vol. 12, pp. 3607- 3617 ,(1993) , 10.1002/J.1460-2075.1993.TB06034.X
Akila Mayeda, Adrian R. Krainer, Preparation of Hela Cell Nuclear and Cytosolic S100 Extracts for In Vitro Splicing RNA-Protein Interaction Protocols. ,vol. 118, pp. 309- 314 ,(1999) , 10.1385/1-59259-676-2:309
A. R. Krainer, J. F. Caceres, Mammalian pre-mRNA splicing factors IRL Press at Oxford University Press. ,(1997)
Akila Mayeda, Adrian R. Krainer, Mammalian In Vitro Splicing Assays RNA-Protein Interaction Protocols. ,vol. 118, pp. 315- 321 ,(1999) , 10.1385/1-59259-676-2:315
Doug Irvine, Craig Tuerk, Larry Gold, SELEXION. Systematic evolution of ligands by exponential enrichment with integrated optimization by non-linear analysis. Journal of Molecular Biology. ,vol. 222, pp. 739- 761 ,(1991) , 10.1016/0022-2836(91)90509-5
H Tian, R Kole, Selection of novel exon recognition elements from a pool of random sequences. Molecular and Cellular Biology. ,vol. 15, pp. 6291- 6298 ,(1995) , 10.1128/MCB.15.11.6291