Molecular monitoring of chronic myeloid leukemia: international standardization of BCR-ABL1 quantitation.

摘要: The BCR-ABL1 translocation is a hallmark of chronic myeloid leukemia. Because patients treated with imatinib and other tyrosine kinase inhibitors achieve lower levels detectable disease, quantitation transcripts quantitative RT-PCR has become an essential tool in leukemia monitoring. prognostic significance molecular responses was recently established by large-scale clinical trials. Achieving defined on the International Scale within specific time frames important measure for assessing patient response probability relapse progression. However, extensive variation procedures reporting makes it difficult to interpret these results. More important, lack standardization, particularly United States, prevents comparison individual results data from trials, which thereby prohibits meaningful use such direction care. In this article, we will present overview trial discoveries that drive need review most updated monitoring guidelines National Comprehensive Cancer Network, highlight recommendations laboratory practice regarding internal controls reference materials. Finally, provide update recent efforts standardization using Scale.