Diastereomeric selectivity of carbon-coated zirconia reversed-phase liquid chromatographic media.

摘要: The determination of enantiomeric excess, that is, the relative amount any pair optical antipodes, constitutes a integral part work analytical and synthetic chemists involved in natural products research or pharmaceutical development. Mosher's reagent [α-methoxy-α-(trifluoromethyl)phenylacetyl chloride] has evolved into major tool for absolute configuration by NMR. We report here on separation diastereomers formed derivatizing enantiomers with reagent. have shown reversed-phase liquid−solid adsorption chromatography carbon surfaces frequently gives considerably superior resolution diastereomeric pairs than does RPLC conventional bonded phases. improved results from very high sensitivity solid to geometric organization solute rather differences column efficiency. compare pharmaceutically biologically important stereoisomeric mixtures, including (±...