Icariin Promotes the Osteogenesis of Bone Marrow Mesenchymal Stem Cells through Regulating Sclerostin and Activating the Wnt/β-Catenin Signaling Pathway.

摘要: Osteoporosis (OP) is a metabolic disease characterized by decreased bone mass and increased risk of fragility fractures, which significantly reduces the quality life. Stem cell-based therapies, especially using marrow mesenchymal stem cells (BMSCs), are promising strategy for treating OP. Nevertheless, survival differentiation rates transplanted BMSCs low, limits their therapeutic efficiency. Icariin (ICA) traditional Chinese medicine formulation that prescribed tonifying kidneys. It also promotes proliferation osteogenic BMSCs, although specific mechanism remains unclear. Based on our previous research, we hypothesized ICA formation via sclerostin/Wnt/β-catenin signaling pathway. We isolated rat transfected them with sclerostin gene (SOST) overexpressing or knockdown constructs assessed induction in presence absence ICA. Sclerostin inhibited BMSC differentiation, whereas not only number viable but enhanced ALP activity calcium nodules during induction. In addition, genes including Runx2, β-catenin, c-myc as well antioxidant factors (Prdx1, Cata, Nqo1) were downregulated restored treatment. Mechanistically, exerted these effects activating Wnt/β-catenin conclusion, can promote situ therefore may enhance efficiency transplantation