Detection of SNPs of T2DM susceptibility genes by a ligase detection reaction-fluorescent nanosphere technique.
作者： Yan Chen , Ying Zhao , Yan-Bo Li , Yan-Jun Wang , Gui-Zhen Zhang
关键词: Molecular biology 、 Molecular Inversion Probe 、 DNA sequencing 、 Multiplex polymerase chain reaction 、 SNP genotyping 、 Genetics 、 SNP 、 Biology 、 Locus (genetics) 、 Multiplex 、 Single-nucleotide polymorphism
摘要: Abstract Objective To establish a high throughput, low cost, and simple nanotechnology-based method for the detection of single nucleotide polymorphism (SNP) loci in type 2 diabetes mellitus (T2DM). Methods Multiplex ligase reaction (LDR) amplification was performed using fluorescently labeled magnetic nanosphere-bound upstream LDR probes downstream with unique fluorescent group each SNP locus. The amplified products were separated by nanospheres then scanned fluorescence spectroscopy. Four associated T2DM detected, including rs13866634 locus SLC30A8, rs10811661in CDKN2A/2B, rs1111875 HHEX gene, rs7903146 TCF7L2 gene. genotype also determined DNA sequencing as control. Results genotypes four gene nanosphere-based multiplex consistent results. accuracy rate 100%. Conclusion A based on PCR established simultaneous susceptibility genes.
sci-hub.se 参考文章(20)
Kan-Sen Chou, Chen-Chih Chen, Preparation of Monodispersed Silica Colloids Using Sol-Gel Method: Cosolvent Effect Ceramic Transactions Series. pp. 57- 67 ,(2012) , 10.1002/9781118407820.CH6
F. Barany, Genetic disease detection and DNA amplification using cloned thermostable ligase Proceedings of the National Academy of Sciences of the United States of America. ,vol. 88, pp. 189- 193 ,(1991) , 10.1073/PNAS.88.1.189
Robert Sladek, Ghislain Rocheleau, Johan Rung, Christian Dina, Lishuang Shen, David Serre, Philippe Boutin, Daniel Vincent, Alexandre Belisle, Samy Hadjadj, Beverley Balkau, Barbara Heude, Guillaume Charpentier, Thomas J. Hudson, Alexandre Montpetit, Alexey V. Pshezhetsky, Marc Prentki, Barry I. Posner, David J. Balding, David Meyre, Constantin Polychronakos, Philippe Froguel, A genome-wide association study identifies novel risk loci for type 2 diabetes Nature. ,vol. 445, pp. 881- 885 ,(2007) , 10.1038/NATURE05616
E. P. Carnevale, D. Kouri, J. T. DaRe, D. T. McNamara, I. Mueller, P. A. Zimmerman, A Multiplex Ligase Detection Reaction-Fluorescent Microsphere Assay for Simultaneous Detection of Single Nucleotide Polymorphisms Associated with Plasmodium falciparum Drug Resistance Journal of Clinical Microbiology. ,vol. 45, pp. 752- 761 ,(2007) , 10.1128/JCM.01683-06
L. J. Scott, K. L. Mohlke, L. L. Bonnycastle, C. J. Willer, Y. Li, W. L. Duren, M. R. Erdos, H. M. Stringham, P. S. Chines, A. U. Jackson, L. Prokunina-Olsson, C.-J. Ding, A. J. Swift, N. Narisu, T. Hu, R. Pruim, R. Xiao, X.-Y. Li, K. N. Conneely, N. L. Riebow, A. G. Sprau, M. Tong, P. P. White, K. N. Hetrick, M. W. Barnhart, C. W. Bark, J. L. Goldstein, L. Watkins, F. Xiang, J. Saramies, T. A. Buchanan, R. M. Watanabe, T. T. Valle, L. Kinnunen, G. R. Abecasis, E. W. Pugh, K. F. Doheny, R. N. Bergman, J. Tuomilehto, F. S. Collins, M. Boehnke, A genome-wide association study of type 2 diabetes in Finns detects multiple susceptibility variants. Science. ,vol. 316, pp. 1341- 1345 ,(2007) , 10.1126/SCIENCE.1142382
Werner Stöber, Arthur Fink, Ernst Bohn, Controlled growth of monodisperse silica spheres in the micron size range Journal of Colloid and Interface Science. ,vol. 26, pp. 62- 69 ,(1968) , 10.1016/0021-9797(68)90272-5
Yong-Zhong Wang, Jun-Hua Xiao, Li-Hua Ruan, Hui-Yin Zhang, Min Chen, Xiang-Ke Pu, Hong-Yu Shen, Guo-Xiang Wu, Detection of the rtA181V/T and rtN236T mutations associated with resistance to adefovir dipivoxil using a ligase detection reaction assay Clinica Chimica Acta. ,vol. 408, pp. 70- 74 ,(2009) , 10.1016/J.CCA.2009.07.016
DAVID T. McNAMARA, LAURIN J. KASEHAGEN, BRIAN T. GRIMBERG, JENNIFER COLE-TOBIAN, WILLIAM E. COLLINS, PETER A. ZIMMERMAN, DIAGNOSING INFECTION LEVELS OF FOUR HUMAN MALARIA PARASITE SPECIES BY A POLYMERASE CHAIN REACTION/LIGASE DETECTION REACTION FLUORESCENT MICROSPHERE-BASED ASSAY American Journal of Tropical Medicine and Hygiene. ,vol. 74, pp. 413- 421 ,(2006) , 10.4269/AJTMH.2006.74.413
Norman P Gerry, Nancy E Witowski, Joseph Day, Robert P Hammer, George Barany, Francis Barany, Universal DNA microarray method for multiplex detection of low abundance point mutations. Journal of Molecular Biology. ,vol. 292, pp. 251- 262 ,(1999) , 10.1006/JMBI.1999.3063
Philip Rosenthal, S Nankoberanyi, GW Mbogo, NP Leclair, MD Conrad, P Tumwebaze, S Tukwasibwe, MR Kamya, J Tappero, SL Nsobya, PJ Rosenthal, Validation of the ligase detection reaction fluorescent microsphere assay for the detection of Plasmodium falciparum resistance mediating polymorphisms in Uganda Malaria Journal. ,vol. 13, pp. 95- 95 ,(2014) , 10.1186/1475-2875-13-95