Scanning and transmission electron microscopic study of adherence of Escherichia coli O103 enteropathogenic and/or enterohemorrhagic strain GV in enteric infection in rabbits.

摘要: Abstract The GV strain (serotype O103:H2:K-), originally isolated from a diarrheic rabbit, is an enteropathogenic Escherichia coli that produces diarrhea without synthesizing the classical enterotoxins and not invasive. This characterized by 117-kb plasmid (pREC-1). Histological study of gut scanning electron microscopy transmission was performed on strain, derivative cured pREC-1, transconjugants obtained transfer pREC-1 to nonpathogenic strains E. K-12 6100, belonging O103 serogroup. The adhered epithelial cells ileum large intestine, whereas did not. Transfer or 6100 allowed bacteria attach intestinal mucosa in same manner as wild-type strain. Thus, seems play important role attachment colonization tract rabbits serogroup O103. Scanning showed numerous attached together closely associated with villi. Transmission revealed effacing lesions characteristic strains: microvilli cuplike projections (pedestal formations) acute inflammatory hemorrhagic response. In contrast results reported for rabbit pathogenic O15 strains, it appeared Peyer's patches were involved early stages infection may represent model virulence effects enterohemorrhagic strains.