INTERLEUKIN-1β ISOLATED FROM A MARINE FISH REVEALS UP-REGULATED EXPRESSION IN MACROPHAGES FOLLOWING ACTIVATION WITH LIPOPOLYSACCHARIDE AND LYMPHOKINES
作者： Pablo Pelegrı́n , Jesús Garcı́a-Castillo , Victoriano Mulero , José Meseguer
关键词: Exon 、 Coding region 、 Open reading frame 、 Polyadenylation 、 Vibrio anguillarum 、 Gene 、 Amino acid 、 Untranslated region 、 Molecular biology 、 Biology
摘要: Abstract The gilthead seabream IL-1β gene consists of five exons/four introns. complete coding sequence contains a 102 bp 5′ untranslated region (UTR), single open reading frame 762 bp which translates into 253 amino acid molecule, and 407 bp 3′UTR with polyadenylation signal 14 nucleotides upstream the poly(A)tail. has highest degree nucleotide (61.7%) (53%) identity trout sequences. message was detected by RT-PCR in head-kidney, blood, spleen, liver, gill peritoneal exudate both non-infected Vibrio anguillarum -challenged fish. More importantly, highly expressed purified macrophage monolayers up-regulated lipopolysaccharide lymphocyte-derived macrophage-activating factor stimulation.
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