Comparative analysis of development‐related gene expression in mouse preimplantation embryos with different developmental potential
作者： Li XiangPing Li XiangPing , Y Kato , Y Tsunoda
DOI: 10.1002/MRD.20346
关键词: Andrology 、 Biology 、 Homeobox protein NANOG 、 Embryo 、 Gene expression 、 Blastomere 、 Regulation of gene expression 、 SOX2 、 Embryonic stem cell 、 Blastocyst 、 Molecular biology
摘要: The potential of embryonic and somatic cell nuclear-transferred (NT) mouse oocytes to develop into young is low compared with bovine NT oocytes. To examine the reasons for developmental oocytes, we analyzed gene expression patterns six development-related genes (Oct4, Nanog, Stat3, stella, FGF4, Sox2) during preimplantation development in manipulated different potentials using real-time polymerase chain reaction (PCR) methods. were parthenogenetically activated stem cell, cumulus morula blastomere vitro-cultured vivo-recovered embryos. mRNA NT-derived embryos markedly differed from vivo vitro counterparts. Some transcript stem-cell resembled those parthenogenetic Of developmentally important transcripts examined embryos, four had a downregulated pattern at blastocyst stage. Our findings indicate that abnormal correlate young. Although more detailed information required, Sox2 blastocysts seems closely
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