Metagenomic analysis reveals the prevalence of biodegradation genes for organic pollutants in activated sludge.

作者: Hua Fang , Lin Cai , Yunlong Yu , Tong Zhang

DOI: 10.1016/J.BIORTECH.2012.11.054

关键词: ProteobacteriaMicrobiologyPollutantBacteroidetesMetagenomicsSewage treatmentBiologyBiodegradationFood scienceActinobacteriaActivated sludge

摘要: The abundance, diversity, and distribution of biodegradation genes (BDGs) phenol degradation (PDGs) in activated sludge (AS) from two wastewater treatment plants (WWTPs) at different sampling times were assessed by metagenomic analysis using a total 15 datasets derived Illumina high-throughput sequencing BLAST comparisons to BDGs PDGs databases. results showed that the abundance (0.015-0.030%) diversity AS varied with WWTP times. p450 pmo most abundant subgroups, respectively. MG-RAST revealed 87 detected bacterial genera potentially capable degrading pollutants mostly affiliated Proteobacteria (59.8%), Bacteroidetes (17.2%), Actinobacteria (9.2%). Mycobacterium, belonging Actinobacteria, was found be genus (23.4%). This method could used monitor an AS's ability for organic evaluate its efficiency.

参考文章(36)
Ren-Ren Wu, Zhi Dang, Xiao-Yun Yi, Chen Yang, Gui-Ning Lu, Chu-Ling Guo, Cong-Qiang Liu, The effects of nutrient amendment on biodegradation and cytochrome P450 activity of an n-alkane degrading strain of Burkholderia sp. GS3C. Journal of Hazardous Materials. ,vol. 186, pp. 978- 983 ,(2011) , 10.1016/J.JHAZMAT.2010.11.095
Tamás Felföldi, Anna J Székely, Róbert Gorál, Katalin Barkács, Gergely Scheirich, Judit András, Anikó Rácz, Károly Márialigeti, None, Polyphasic bacterial community analysis of an aerobic activated sludge removing phenols and thiocyanate from coke plant effluent Bioresource Technology. ,vol. 101, pp. 3406- 3414 ,(2010) , 10.1016/J.BIORTECH.2009.12.053
Susanne Schneiker, Vítor AP Martins dos Santos, Daniela Bartels, Thomas Bekel, Martina Brecht, Jens Buhrmester, Tatyana N Chernikova, Renata Denaro, Manuel Ferrer, Christoph Gertler, Alexander Goesmann, Olga V Golyshina, Filip Kaminski, Amit N Khachane, Siegmund Lang, Burkhard Linke, Alice C McHardy, Folker Meyer, Taras Nechitaylo, Alfred Pühler, Daniela Regenhardt, Oliver Rupp, Julia S Sabirova, Werner Selbitschka, Michail M Yakimov, Kenneth N Timmis, Frank-Jörg Vorhölter, Stefan Weidner, Olaf Kaiser, Peter N Golyshin, Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis Nature Biotechnology. ,vol. 24, pp. 997- 1004 ,(2006) , 10.1038/NBT1232
F Meyer, D Paarmann, M D'Souza, R Olson, EM Glass, M Kubal, T Paczian, A Rodriguez, R Stevens, A Wilke, J Wilkening, RA Edwards, The Metagenomics RAST Server: A Public Resource for the Automatic Phylogenetic and Functional Analysis of Metagenomes BMC Bioinformatics. ,vol. 9, pp. 386- 386 ,(2008) , 10.1186/1471-2105-9-386
Rachel Mackelprang, Mark P. Waldrop, Kristen M. DeAngelis, Maude M. David, Krystle L. Chavarria, Steven J. Blazewicz, Edward M. Rubin, Janet K. Jansson, Metagenomic analysis of a permafrost microbial community reveals a rapid response to thaw Nature. ,vol. 480, pp. 368- 371 ,(2011) , 10.1038/NATURE10576
Cédric Malandain, Françoise Fayolle-Guichard, Timothy M. Vogel, Cytochromes P450-mediated degradation of fuel oxygenates by environmental isolates. FEMS Microbiology Ecology. ,vol. 72, pp. 289- 296 ,(2010) , 10.1111/J.1574-6941.2010.00847.X
Erik Kristiansson, Jerker Fick, Anders Janzon, Roman Grabic, Carolin Rutgersson, Birgitta Weijdegård, Hanna Söderström, D. G. Joakim Larsson, Pyrosequencing of Antibiotic-Contaminated River Sediments Reveals High Levels of Resistance and Gene Transfer Elements PLoS ONE. ,vol. 6, pp. e17038- ,(2011) , 10.1371/JOURNAL.PONE.0017038
Michael Bunge, Lorenz Adrian, Angelika Kraus, Matthias Opel, Wilhelm G. Lorenz, Jan R. Andreesen, Helmut Görisch, Ute Lechner, Reductive dehalogenation of chlorinated dioxins by an anaerobic bacterium Nature. ,vol. 421, pp. 357- 360 ,(2003) , 10.1038/NATURE01237
Lynne Whan, Hywel J. Ball, Irene R. Grant, Michael T. Rowe, Occurrence of Mycobacterium avium subsp. paratuberculosis in Untreated Water in Northern Ireland Applied and Environmental Microbiology. ,vol. 71, pp. 7107- 7112 ,(2005) , 10.1128/AEM.71.11.7107-7112.2005