Whey microbeads as a matrix for the encapsulation and immobilisation of riboflavin and peptides

作者: Graham J O’Neill , Thelma Egan , Jean Christophe Jacquier , Michael O’Sullivan , E Dolores O’Riordan

DOI: 10.1016/J.FOODCHEM.2014.03.002

关键词: PeptideHydrophobic effectAmino acidChemistryRiboflavinChromatographyWhey protein

摘要: Abstract Whey microbeads manufactured using a cold-set gelation process, have been used to encapsulate bioactives. In this study whey were riboflavin 2 methods. Riboflavin was added the microbead forming solution however diffusional losses of occurred during subsequent bead preparation. To overcome loss, second approach ‘load’ by soaking in assessed. Significantly (p ⩽ 0.05) higher concentrations obtained ‘loaded’ (361 mg/L) compared (48 mg/L). uptake shown be via partition process. As partitioning is often driven hydrophobic interactions amino acids and peptides varying hydrophobicities examined. The % encapsulation increased with increasing molecule hydrophobicity maximum 89% encapsulation. are well suited act as sorbents for

参考文章(33)
Whitney L. Stoppel, Joseph C. White, Sarena D. Horava, Surita R. Bhatia, Susan C. Roberts, Transport of biological molecules in surfactant–alginate composite hydrogels Acta Biomaterialia. ,vol. 7, pp. 3988- 3998 ,(2011) , 10.1016/J.ACTBIO.2011.07.009
S. F. Baranovskii, P. A. Bolotin, Association of riboflavin, caffeine, and sodium salicylate in aqueous solution Journal of Applied Spectroscopy. ,vol. 74, pp. 211- 218 ,(2007) , 10.1007/S10812-007-0033-8
Lingyun Chen, Muriel Subirade, Effect of preparation conditions on the nutrient release properties of alginate–whey protein granular microspheres European Journal of Pharmaceutics and Biopharmaceutics. ,vol. 65, pp. 354- 362 ,(2007) , 10.1016/J.EJPB.2006.10.012
Géraldine Hébrard, Stéphanie Blanquet, Eric Beyssac, Gabriel Remondetto, Muriel Subirade, Monique Alric, Use of whey protein beads as a new carrier system for recombinant yeasts in human digestive tract Journal of Biotechnology. ,vol. 127, pp. 151- 160 ,(2006) , 10.1016/J.JBIOTEC.2006.06.012
Maria Palasis, Stevin H. Gehrke, Permeability of responsive poly (N-isopropylacrylamide) gel to solutes Journal of Controlled Release. ,vol. 18, pp. 1- 11 ,(1992) , 10.1016/0168-3659(92)90205-6
Jwala Renukuntla, Aswani Dutt Vadlapudi, Ashaben Patel, Sai H.S. Boddu, Ashim K. Mitra, Approaches for Enhancing Oral Bioavailability of Peptides and Proteins International Journal of Pharmaceutics. ,vol. 447, pp. 75- 93 ,(2013) , 10.1016/J.IJPHARM.2013.02.030
F. Hachem, B.A. Andrews, J.A. Asenjo, Hydrophobic partitioning of proteins in aqueous two-phase systems Enzyme and Microbial Technology. ,vol. 19, pp. 507- 517 ,(1996) , 10.1016/S0141-0229(96)80002-D