β-Lactoglobulin enhances the uptake of free palmitate by hepatocyte monolayers: the relative importance of diffusion and facilitated dissociation

摘要: We compared the uptake of bound palmitate by rat hepatocytes to its polyethylene using β-lactoglobulin (BLG) as binding protein. The experiments were designed supply a direct measure protein-dependent change in diffusive conductance extracellular fluid without determining diffusion coefficients for free and fatty acid or off-rate constant protein binding. Rate-limiting dissociation stirred phase was excluded. results obtained with BLG are strikingly similar those previously albumin provide additional circumstantial evidence that when fraction is small, partially driven concentration acid. Because this phenomenon not specific protein, it may reflect exchange ligand between putative transport hepatocyte plasma membrane.Key words: codiffusion, stagnant layer, pro...