Correlations between Mitofusin 2 Expression in Fibroblasts and Pelvic Organ Prolapse: An In vitro Study.

摘要: Background: Both Mitofusin 2 (Mfn2) and pelvic organ prolapse (POP) are related to aging. The aim of the present study was investigate variations Mfn2 expression in uterosacral ligaments patients with and/or without POP their correlations procollagen. Methods: Fibroblasts were cultured using tissue specimens that harvested from non-POP (NPOP) (n = 10 for each group) September 2016 December 2016. Cell Counting Kit-8 (CCK-8) assay used compare differences cell proliferation between two groups. Relative quantitative reverse transcription-polymerase chain reaction Western blotting assays employed assess mRNA protein levels procollagen 1A1/1A2/3A1 changes assessed following downregulation group RNAi. data independent sample t-test or general linear model univariate analysis SPSS 13.0 software. Results: The results CCK-8 indicated viability significantly lower compared NPOP (td5,7,9,11= −5.925, −6.851, −9.129, −9.661, respectively, all P< 0.001, D5 D11). fibroblasts higher those (mRNA: t 2.425, P= 0.032; protein: 2.392, 0.037, respectively), whereas only t= −2.165, P1A1 0.041; −2.741, P1A2 0.026; −2.147, P3A1 0.045, respectively; −2.418, 0.029; −2.405, 0.033; −2.470, 0.012, respectively). increased Mfn2. Conclusions: The rate group. In fibroblasts, increased, while decreased. Key words: Cell Culture; Mitofusin 2; Pelvic Organ Prolapse; Procollagen