Fusion between Sendai virus envelopes and biological membranes. The use of fluorescent probes for quantitative estimation of virus-membrane fusion.

摘要: The fluorescent probes, N-4-nitrobenzo-2-oxa-1,3-diazole-phosphatidylethanolamine and lissamine-rhodamine-B-sulfonylphosphatidylethanolamine, were inserted at the appropriate surface density into membranes of reconstituted Sendai virus envelopes, thus allowing transfer energy between probes. In addition, only molecule was viral resulting in self-quenching. Incubation fluorescent, envelopes with human erythrocyte ghosts resulted either reduction efficiency or fluorescence dequenching. No dequenching observed when incubated glutaraldehyde-fixed desialized ghosts. Similarly, no change value nonfusogenic, These results clearly show that is due to virus-membrane fusion not lipid-lipid exchange. carrying N-4-nitrobenzo-2-oxa-1,3-diazolephosphatidylethanolamine, cultured cells also a significant measurable However, incubation hepatoma tissue culture dequenching, degree which about 50% fusogenic, envelopes. It therefore possible that, addition fusion, endocytosis as well.