Neurokinin 3 receptor forms a complex with acetylated histone H3 and H4 in hypothalamic neurons following hyperosmotic challenge
作者： Francis W. Flynn , Dane D. Jensen , Amit Thakar , Xihui Xu , Steven W. Flynn
DOI: 10.1152/AJPREGU.00254.2011
关键词: Transcriptionally active chromatin 、 Hypothalamus 、 Nucleus 、 Internal medicine 、 Endocrinology 、 Receptor 、 Immunogold labelling 、 Cell biology 、 Cytoplasm 、 Histone H4 、 Biology 、 Cell nucleus
摘要: The neurokinin 3 receptor (NK3R) is a G protein-coupled that expressed in brain and highly by magnocellular vasopressinergic neurons both the paraventricular (PVN) supraoptic nuclei (SON) of hypothalamus. Hyperosmolarity causes ligand-mediated internalization NK3Rs to cytoplasm PVN neurons. This activation-dependent pathway presumed be means directly transmit synaptic signals from cell membrane nucleus. present study evaluated vivo subnuclear domains associate with NK3R. Rats were administered 2 M NaCl (intragastric) or no intragastric load, 40 min later, was dissected isolated. Using double-immuno-transmission electron microscopy (TEM), we show that, compared controls, hyperosmolarity significant increase NK3R Immunogold beads nucleus Furthermore, spatially colocalized histone H4 acetylated isolated rats NaCl, but not control rats. Next, coimmunoprecipitation experiments showed H4, as well H3, pulled down nuclear enriched fraction treated In response hyperosmolarity, transported associates transcriptionally active chromatin, where it may influence transcription genes.
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