Bioengineered silver nanoparticles capped with bovine serum albumin and its anticancer and apoptotic activity against breast, bone and intestinal colon cancer cell lines.
作者： Shahnaz Majeed , Farah Hanani Binti Aripin , Nur Syafiqah Binti Shoeb , Mohammed Danish , MN Mohamad Ibrahim
DOI: 10.1016/J.MSEC.2019.04.041
关键词: Silver nanoparticle 、 Materials science 、 Bovine serum albumin 、 Nuclear chemistry 、 Protein degradation 、 Lactate dehydrogenase 、 Albumin 、 Silver nitrate 、 Acridine orange 、 Fragmentation (cell biology)
摘要: Abstract The aim of the current study was to biosynthesize silver nanoparticles (AgNPs) from bacterial strain Bacillus cereus (ATCC 14579) extracellularly. When extract challenged with 1 mM nitrate (AgNO3) color changed into brown confirms formation nanoparticles. These were capped bovine serum albumin (BSA). UV- visible spectroscopy showed absorption peak at 420 nm indicates AgNPs. Fourier Infra –red (FTIR) attenuated total reflection (ATR) amide and amine group associated AgNPs that stabilizes Energy dispersive x-ray (EDX) a strong presence silver. Thermo gravimetric analysis (TGA) used determine protein degradation less higher temperature stability Transmission electron microscopy (TEM) are well dispersed spherical, 5.37 nm 17.19 whereas coated size ranges 11.26 nm 23.85 nm. anticancer effect (cAgNPs) IC50 value against breast cancer MCF-7 80 μg/mL, intestinal colon HCT- 116 60 μg/mL, bone osteosarcoma MG-63 cell line80 μg/mL while normal fibroblast cells 3T3 140 μg/mL. Lactate dehydrogenase assay (LDH) toxicity on MCF-7, HCT-116, cells. apoptotic clearly blebbing membrane, chromatin condensation due production reactive oxygen species (ROS) by ethidium bromide acridine orange dual staining method. DNA complete fragmentation treated when compared control
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