Descriptive Comparison of ELISAs for the Detection of Toxoplasma gondii Antibodies in Animals: A Systematic Review.

摘要: Toxoplasma gondii is the zoonotic parasite responsible for toxoplasmosis in warm-blooded vertebrates. This systematic review compares and evaluates available knowledge on enzyme-linked immunosorbent assays (ELISAs), their components, performance detecting T. antibodies animals. Four databases were searched published scientific studies ELISA, 57 articles included. Overall, indirect (95%) in-house (67%) ELISAs most used types of test among examined, but ‘ID Screen® Toxoplasmosis Indirect Multi-species’ was common commercially tests. Varying diagnostic (sensitivity specificity) Kappa agreements observed depending type sample (serum, meat juice, milk), antigen (native, recombinant, chimeric) antibody-binding reagents used. Combinations recombinant chimeric antigens resulted better than native or single antigens. Protein A/G appeared to be useful IgG a wide range animal species due its non-species-specific binding. One study reported cross-reactivity, with Hammondia hammondi Eimeria spp. first descriptively compare detection across different species.