Hepatoprotective efficacy of certain flavonoids against microcystin induced toxicity in mice

摘要: Toxic cyanobacteria (blue-green algae) water blooms have become a serious problem in several industrialized areas of the world. Microcystin-LR (MC-LR) is cyanobacterial heptapeptide that represents acute and chronic hazards to animal human health. Identification suitable chemprotectants against microcystin essential considering health hazards. In present study, we evaluated protective efficacy three flavanoids namely quercetin (200 mg/kg), silybin (400 morin mg/kg)] pretreatment toxicity (0.75 LD(50), 57.5 microg/kg) mice. Various biochemical variables were measured study recovery profile protected animals at 1- 3-days post-toxin treatment. The serum alanine amino transferase (ALT) shows 17-fold increase MC-LR treated compared with control group 1 day. showed decrease level ALT but still higher than group. No significant protection was observed aspartate aminotransaminase (AST) lactate dehydrogenase (LDH) levels flavanoid-treated groups 1-day post-treatment. But 3 days, AST normalized values, LDH significantly changes glutathione peroxidase reduced both 3-day postexposure. catalase activity quercetin-treated protein phosphatase inhibited control. pretreated after At PPAse reversed values all groups. Immunoblotting analysis microcystin-PPAse adduct liver tissues toxin-treated as well mice even days. results this show flavanoids, quercetin, silybin, could reverse hepatotoxic effects vivo.