A preliminary in vitro study into the use of IL-1Ra gene therapy for the inhibition of intervertebral disc degeneration.
作者： Christine L. Le Maitre , Anthony J. Freemont , Judith A. Hoyland
DOI: 10.1111/J.0959-9673.2006.00449.X
关键词: Genetic transfer 、 Pathology 、 In vitro 、 Intervertebral disc 、 Ex vivo 、 Gene expression 、 Biology 、 Intervertebral disk 、 Molecular biology 、 Viral vector 、 Genetic enhancement
摘要: Conventional therapies for low back pain (LBP) are purely symptomatic and do not target the cause of LBP, which in approximately 40% cases is caused by degeneration intervertebral disc (DIVD). Targeting to inhibit process would be a potentially valuable treatment LBP. There increasing evidence role IL-1 DIVD. A natural inhibitor exists, IL-1Ra, an ideal molecular inhibiting IL-1-mediated effects involved DIVD In this study, feasibility ex vivo gene transfer IL-1Ra IVD was investigated. Monolayer alginate cultures normal degenerate human (IVD) cells were infected with adenoviral vector carrying (Ad-IL-1Ra) protein production measured using enzyme-linked immunosorbent assay. The ability these also addition, Ad-IL-1Ra injected into tissue explants discs assessed. This demonstrated that both nucleus pulposus annulus fibrosus produced elevated levels prolonged time periods, resistant IL-1. When explants, expression increased maintained 2 weeks investigation. vitro study has shown use feasible therapy inhibition events during degeneration.
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