Towards an integrated microfluidic device for spaceflight clinical diagnostics
作者： Daniel J. Marchiarullo , Ji Y. Lim , Zalman Vaksman , Jerome P. Ferrance , Lakshmi Putcha
DOI: 10.1016/J.CHROMA.2008.05.031
关键词: Solid phase extraction 、 Chromatography 、 Resolution (mass spectrometry) 、 Column chromatography 、 Capillary electrophoresis 、 Extraction (chemistry) 、 Metabolite 、 Small molecule 、 Sample preparation 、 Chemistry 、 Organic chemistry 、 Analytical chemistry 、 Biochemistry 、 General Medicine
摘要: A microchip-based solid-phase extraction method for biological fluid small molecule analysis has been developed. Using a commercially available copolymer packed into microchip channel, and preconcentration of 2,3-dihydroxybenzoic acid (DHBA) 2,5-DHBA from saliva was achieved. The metabolites, formed salicylic by reactive oxygen species, can be used as markers oxidative stress. results show high recovery both metabolites (>90±15% spiked saliva) with an 80-fold concentration enhancement possible. eluent is directly analyzed using capillary electrophoresis, good resolution the two metabolites. This study demonstrates feasibility future integrated microdevices spaceflight biomarker analysis.
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