Self-assembled nanocomplexes of anionic pullulan and polyallylamine for DNA and pH-sensitive intracellular drug delivery

摘要: The amalgamation of chemotherapy and gene therapy is promising treatment option for cancer. In this study, novel biocompatible self-assembled nanocomplexes (NCs) between carboxylmethylated pullulan t335 (CMP) with polyallylamine (CMP–PAA NCs) were developed plasmid DNA (pDNA) pH-sensitive doxorubicin (DOX) delivery. DOX was conjugated to CMP (DOX–CMP) via hydrazone confirmed by FTIR 1H-NMR. vitro release studies DOX–CMP conjugate showed 23 85 % after 48 h at pH 7.4 (physiological pH) 5 (intracellular/tumoral pH), respectively. CMP–PAA NCs or DOX–CMP–PAA into a nanosized (<250 nm) spherical shape as DLS TEM. hemolysis cytotoxicity study indicated that the did not show in comparison plain polyallylamine. Gel retardation assay complete binding pDNA 1:2 weight ratio. NCs/pDNA significantly higher transfection HEK293 cells compared PAA/pDNA complexes. Confocal imaging demonstrated successful cellular uptake cells. Thus, hold great potential targeted intratumoral drug