Structural characterization of the human platelet-derived growth factor A-chain cDNA and gene: alternative exon usage predicts two different precursor proteins.

摘要: The human platelet-derived growth factor (PDGF) A-chain locus was characterized by restriction endonuclease analysis, and the nucleotide sequence of its exons determined. Seven were identified, spanning approximately 22 kilobase pairs genomic DNA. Alternative exon usage, identified cDNA cloning, occurs in a glioblastoma cell line may give rise to two types precursors with different C termini. exon-intron arrangement similar that PDGF B-chain/sis seemed divide precursor proteins into functional domains. Southern blot analysis DNA showed single gene present genome.