Involvement of miR-106b in tumorigenic actions of both prolactin and estradiol.
作者： Kuan-Hui Ethan Chen , Karissa Bustamante , Vi Nguyen , Ameae M. Walker
DOI: 10.18632/ONCOTARGET.16755
关键词: Endocrinology 、 MAPK/ERK pathway 、 Internal medicine 、 PI3K/AKT/mTOR pathway 、 Cell growth 、 Prolactin cell 、 Signal transduction 、 Cancer research 、 Cancer cell 、 Protein kinase B 、 Prolactin 、 Biology
摘要: Prolactin promotes a variety of cancers by an array different mechanisms. Here, we have investigated prolactin's inhibitory effect on expression the cell cycle-regulating protein, p21. Using miRNA array, identified number miRNAs upregulated prolactin treatment, but one in particular that was strongly induced and predicted to bind 3'UTR p21 mRNA, miR-106b. By creating mRNA 3'UTR-luciferase construct, demonstrated degradation construct response human breast, prostate ovarian cancer lines. Increased miR-106b replicated, anti-miR-106b counteracted, effects levels, proliferation breast (T47D) (PC3) cells. also stimulated migration very epithelioid T47D line. contrast, dramatically decreased mesenchymal markers, SNAIL-2, TWIST-2, VIMENTIN, FIBRONECTIN. signaling pathway inhibitors induction determined be mediated through MAPK/ERK PI3K/Akt pathways not Jak2/Stat5 both PC3 activation activates ERα absence ligand. 17β-estradiol promoted lines pre-incubation estrogen antagonist, Fulvestrant, blocked ability induce construct-degrading activity. Together, these data support convergence miR-106b-elevating at ERα.
sci-hub.st 参考文章(81)
Fengqin Shi, Xinyi Chen, Alan Fu, Johnni Hansen, Richard Stevens, Anne Tjonneland, Ulla B Vogel, Tongzhang Zheng, Yong Zhu, None, Aberrant DNA methylation of miR-219 promoter in long-term night shiftworkers Environmental and Molecular Mutagenesis. ,vol. 54, pp. 406- 413 ,(2013) , 10.1002/EM.21790
Kumaravel Somasundaram, Hongbing Zhang, Yi-Xin Zeng, Yariv Houvras, Yi Peng, Hongxiang Zhang, Gen Sheng Wu, Jonathan D. Licht, Barbara L. Weber, Wafik S. El-Deiry, Arrest of the cell cycle by the tumour-suppressor BRCA1 requires the cdk-inhibitor p21waf1/cip1 Nature. ,vol. 389, pp. 187- 190 ,(1997) , 10.1038/38291
Tomohiro Yonezawa, Kuan-Hui (Ethan) Chen, Mrinal K. Ghosh, Lorena Rivera, Riva Dill, Lisa Ma, Pedro A. Villa, Mitsumori Kawaminami, Ameae M. Walker, Anti-metastatic outcome of isoform-specific prolactin receptor targeting in breast cancer Cancer Letters. ,vol. 366, pp. 84- 92 ,(2015) , 10.1016/J.CANLET.2015.06.010
Bruno M Simões, Denis G Alferez, Sacha J Howell, Robert B Clarke, The role of steroid hormones in breast cancer stem cells Endocrine-related Cancer. ,vol. 22, pp. 177- 186 ,(2015) , 10.1530/ERC-15-0350
Swati Dhar, Avinash Kumar, Agnes M Rimando, Xu Zhang, Anait S Levenson, None, Resveratrol and pterostilbene epigenetically restore PTEN expression by targeting oncomiRs of the miR-17 family in prostate cancer Oncotarget. ,vol. 6, pp. 27214- 27226 ,(2015) , 10.18632/ONCOTARGET.4877
C. Benson Kuo, Xiaolei Xu, Eva Kreye, Ameae M. Walker, A molecular mimic of phosphorylated prolactin markedly reduced tumor incidence and size when DU145 human prostate cancer cells were grown in nude mice. Cancer Research. ,vol. 61, pp. 6098- 6104 ,(2001)
YenHao Chen, KuangTzu Huang, KuanHui E Chen, Ameae M Walker, None, Prolactin and estradiol utilize distinct mechanisms to increase serine-118 phosphorylation and decrease levels of estrogen receptor α in T47D breast cancer cells Breast Cancer Research and Treatment. ,vol. 120, pp. 369- 377 ,(2010) , 10.1007/S10549-009-0400-7
R S Hudson, M Yi, D Esposito, S A Glynn, A M Starks, Y Yang, A J Schetter, S K Watkins, A A Hurwitz, T H Dorsey, R M Stephens, C M Croce, S Ambs, MicroRNA-106b-25 cluster expression is associated with early disease recurrence and targets caspase-7 and focal adhesion in human prostate cancer. Oncogene. ,vol. 32, pp. 4139- 4147 ,(2013) , 10.1038/ONC.2012.424
Hongzi Liang, Leo Studach, Ronald L. Hullinger, Jun Xie, Ourania M. Andrisani, Down-regulation of RE-1 silencing transcription factor (REST) in advanced prostate cancer by hypoxia-induced miR-106b~25. Experimental Cell Research. ,vol. 320, pp. 188- 199 ,(2014) , 10.1016/J.YEXCR.2013.09.020
M. B. Datto, Y. Li, J. F. Panus, D. J. Howe, Y. Xiong, X. F. Wang, Transforming growth factor beta induces the cyclin-dependent kinase inhibitor p21 through a p53-independent mechanism Proceedings of the National Academy of Sciences of the United States of America. ,vol. 92, pp. 5545- 5549 ,(1995) , 10.1073/PNAS.92.12.5545