Poly(ADP-ribosyl)ation: a posttranslational protein modification linked with genome protection and mammalian longevity.

摘要: Poly(ADP-ribosyl)ation is a posttranslational modification of nuclear proteins catalysed by the 113-kDa enzyme poly(ADP-ribose) polymerase-1 (PARP-1) and, to lesser extent, several other recently described polypeptides. The catalytic function PARP-1 directly stimulated DNA strand breaks, thus making poly(ADP-ribosyl)ation one immediate cellular responses oxidative and types damage. plays an important role in recovery proliferating cells from certain damage, this has been linked mechanistically with involvement base-excision repair. Furthermore activity necessary maintain genomic stability under conditions genotoxic stress actually key regulator alkylation-induced sister-chromatid exchange formation, imposing control that strictly negative commensurate level. Finally, there positive correlation between capacity mononuclear leukocytes various mammalian species species-specific life span. Likewise, lymphoblastoid cell lines derived human centenarians display higher than controls. In conclusion, may be viewed as factor responsible for downregulating rate instability events, which are provoked constant attack endogenous exogenous DNA-damaging agents, such way tune them level just appropriate span potential given species.