Partial purification of murine B cell stimulatory factor (BSF)-1.

摘要: BSF-1 was partially purified from serum-free culture supernatants of cells the EL-4 thymoma line, which had been induced 48 hr earlier with 4 beta-phorbol-12 beta-myristate-12 alpha-acetate (PMA). in 10-liter batches adsorbed onto and eluted trimethylsilyl-controlled pore glass beads (TMS-CpG) then subjected to reverse-phase high-performance liquid chromatography (RP-HPLC). The recovery activity by TMS-CpG RP-HPLC ranged 52 55% 187 227%, respectively. specific units per milligram protein approximately 2600 times higher than that supernatant protein. a single isoelectric point 6.3 an apparent m.w. between 18,000 21,700 when analyzed focusing gel filtration-HPLC, ability prepare large amounts rapid efficient procedure should be great help both biochemical immunologic studies this lymphokine.