Fluorescence microscopy techniques for the study of G protein-coupled receptor trafficking.

摘要: Publisher Summary This chapter discusses various methods that have been used to examine G protein-coupled receptors (GPCRs) by fluorescence microscopy. Because many GPCRs exhibit rapid agonist-dependent trafficking, a better understanding of GPCR biology must include knowledge subcellular distribution patterns and the way which these relate regulatory processes such as desensitization resensitization. Receptor localization in absence ligand is also an important aspect characterization. Finally, simple microscopy techniques are invaluable troubleshooting efforts express cloned model cell culture systems. The describes can be trafficking microscopy, both living cells fixed cells. Satisfactory results for most studies may obtained with regular more specifically termed “epifluorescence.” However, confocal eliminates some interference from light outside focal plane, generally producing higher contrast image. planes narrower serial cross-section images acquired (a Z series).