Thermomyces lanuginosus lipase-catalyzed hydrolysis of the lipid cubic liquid crystalline nanoparticles
作者： Justas Barauskas , Hanna Anderberg , Allan Svendsen , Tommy Nylander
DOI: 10.1016/J.COLSURFB.2015.04.052
关键词: Titration 、 Crystallography 、 Nanoparticle 、 Hydrolysis 、 Protonation 、 Micelle 、 Micellar cubic 、 Lipase 、 Phase (matter) 、 Chemistry
摘要: In this study well-ordered glycerol monooleate (GMO)-based cubic liquid crystalline nanoparticles (LCNPs) have been used as substrates for Thermomyces lanuginosus lipase in order to establish the relation between catalytic activity, measured by pH-stat titration, and change morphology nanostructure determined cryogenic transmission electron microscopy synchrotron small angle X-ray diffraction. The initial catalyzed LCNP hydrolysis rate is approximately 25% higher large 350nm compared 190nm particles, which attributed increased number of structural defects on particle surface. At pH 8.0 8.4 bicontinuous Im3m LCNPs transform into "sponge"-like assemblies disordered multilamellar onion-like structures upon exposure lipase. 6.5 7.5 lipolysis induced phase transitions inner core following sequence → reversed hexagonal micellar Fd3m micelles. These phases with negative curvature lipid/water interface were found trigger protonation oleic acid produced during reaction. increase discrete was suggested cause an pKa a larger value observed titration.
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