The CATS (FAM64A) protein is a substrate of the Kinase Interacting Stathmin (KIS).
作者： Leticia Fröhlich Archangelo , Philipp A. Greif , Alexandre Maucuer , Valérie Manceau , Naresh Koneru
DOI: 10.1016/J.BBAMCR.2013.02.004
关键词: UHMK1 、 Cell cycle 、 Kinase 、 Immunoprecipitation 、 Transactivation 、 Biology 、 Molecular biology 、 Stathmin 、 Fusion protein 、 CATS
摘要: Abstract The CATS protein (also known as FAM64A and RCS1) was first identified a novel CALM (PICALM) interactor that influences the subcellular localization of leukemogenic fusion CALM/AF10. is highly expressed in cancer cell lines cycle dependent manner induced by mitogens. considered marker for proliferation, to control metaphase-to-anaphase transition during division. Using bait yeast two-hybrid screen we Kinase Interacting Stathmin (KIS or UHMK1) interacting partner. interaction between KIS confirmed GST pull-down, co-immunopreciptation co-localization experiments. kinase assay showed substrate mapped phosphorylation site serine 131 (S131). Protein expression analysis revealed levels changed cycle-dependent opposite direction levels. In reporter gene able enhance transcriptional repressor activity CATS, independent phophorylation at S131. Moreover, antagonize transactivation capacity CALM/AF10.In summary, our results show interacts with KIS, suggesting regulates function.
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