Topology Engineering of Proteins in Vivo Using Genetically Encoded, Mechanically Interlocking SpyX Modules for Enhanced Stability.
作者： Dong Liu , Wen-Hao Wu , Ya-Jie Liu , Xia-Ling Wu , Yang Cao
DOI: 10.1021/ACSCENTSCI.7B00104
关键词: Recombinant DNA 、 Chemistry 、 Translocon 、 Isopeptide bond 、 Biophysics 、 Protein topology 、 Topology (chemistry) 、 Protein domain 、 Catenane 、 Crystallography 、 Ring (chemistry)
摘要: Recombinant proteins are traditionally limited to linear configuration. Herein, we report in vivo protein topology engineering using highly efficient, mechanically interlocking SpyX modules named AXB and BXA. domains composed of p53dim (X), SpyTag (A), SpyCatcher (B). The guides the intertwining two nascent chains followed by autocatalytic isopeptide bond formation between fulfill interlocking, leading a variety backbone topologies. Direct expression or BXA produces catenanes with distinct ring sizes. containing obtained either as interlocked obligate dimers if interest is fused N- C-terminus modules, star both C-termini. As examples, cellular syntheses (GB1)2 (where GB1 stands for immunoglobulin-binding domain B1 streptococcal G) four-arm elast...
acs.org
core.ac.uk
sci-hub.se 参考文章(57)
Topology and geometry in polymer science Springer New York. ,(1998) , 10.1007/978-1-4612-1712-1
Nikos Hadjichristidis, Akira Hirao, Yasuyuki Tezuka, Filip Du Prez, Complex macromolecular architectures : synthesis, characterization, and self-assembly John Wiley & Sons (Asia) Pte Ltd. ,(2011) , 10.1002/9780470825150
David Whitford, Proteins : structure and function Wiley. ,(2005)
John W Blankenship, Philip E Dawson, Thermodynamics of a Designed Protein Catenane Journal of Molecular Biology. ,vol. 327, pp. 537- 548 ,(2003) , 10.1016/S0022-2836(03)00115-3
Dan W. Urry, Entropic elastic processes in protein mechanisms. I. Elastic structure due to an inverse temperature transition and elasticity due to internal chain dynamics. Journal of Protein Chemistry. ,vol. 7, pp. 1- 34 ,(1988) , 10.1007/BF01025411
B. Zakeri, J. O. Fierer, E. Celik, E. C. Chittock, U. Schwarz-Linek, V. T. Moy, M. Howarth, Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin Proceedings of the National Academy of Sciences of the United States of America. ,vol. 109, pp. 4347- 4348 ,(2012) , 10.1073/PNAS.1115485109
Wim van ’t Hof, Silvie Hansenová Maňásková, Enno C.I. Veerman, Jan G.M. Bolscher, Sortase-mediated backbone cyclization of proteins and peptides. Biological Chemistry. ,vol. 396, pp. 283- 293 ,(2015) , 10.1515/HSZ-2014-0260
Craig J Hawker, Karen L Wooley, The Convergence of Synthetic Organic and Polymer Chemistries Science. ,vol. 309, pp. 1200- 1205 ,(2005) , 10.1126/SCIENCE.1109778
Yi-Tsu Chan, Xiaopeng Li, Jing Yu, Gustavo A. Carri, Charles N. Moorefield, George R. Newkome, Chrys Wesdemiotis, Design, Synthesis, and Traveling Wave Ion Mobility Mass Spectrometry Characterization of Iron(II)– and Ruthenium(II)–Terpyridine Metallomacrocycles Journal of the American Chemical Society. ,vol. 133, pp. 8794- 8804 ,(2011) , 10.1021/JA107307U