Engineering the Escherichia coli outer membrane protein OmpC for metal bioadsorption
作者： Norberto Cruz , Sylvie Le Borgne , Georgina Hernández-Chávez , Guillermo Gosset , Fernando Valle
关键词: Protein engineering 、 Fluorescence microscope 、 Metal 、 Biochemistry 、 Bacteria 、 Epitope 、 Bacterial outer membrane 、 Biology 、 Escherichia coli 、 Cell
摘要: The outer membrane protein, OmpC, from Escherichia coli was used to display metal-binding poly-histidine peptides on the surface of this bacterium. SDS-PAGE analysis protein preparations confirmed expression epitopes inserted in position 162 mature OmpC protein. Display these by epifluorescence microscopy cells bound Ni2+-NTA-agarose beads and metal adsorption experiments. harboring one or two copies binding epitope were able adsorb 3 6 times more Zn2+ (13.8 μmol g−1 cell), Fe3+ (35.3 Ni2+ (9.9 cell) metallic ions than control expressing wild-type OmpC.
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