Clonal Diversity of Chilean Isolates of Enterohemorrhagic Escherichia coli from Patients with Hemolytic-Uremic Syndrome, Asymptomatic Subjects, Animal Reservoirs, and Food Products
作者： Maritza Rios , Valeria Prado , Michele Trucksis , Carolina Arellano , Consuelo Borie
DOI: 10.1128/JCM.37.3.778-781.1999
关键词: Microbiology 、 Disease reservoir 、 Serotype 、 Biology 、 Genotype 、 Typing 、 Virulence 、 Molecular epidemiology 、 Pulsed-field gel electrophoresis 、 Escherichia coli
摘要: To determine clonal relationship among Chilean enterohemorrhagic Escherichia coli (EHEC) strains from different sources (clinical infections, animal reservoirs, and food), 54 EHEC isolates (44 of E. O157, 5 O111, O26) were characterized for virulence genes by colony blot hybridization pulsed-field gel electrophoresis (PFGE). By blotting, 12 genotypes identified the 44 O157 analyzed, which genetic profile stx1+ stx2+ hly+ eae+ was most prevalent. All human that associated with sporadic cases hemolytic-uremic syndrome (HUS) carried both stx1 stx2 toxin-encoding eaeA positive. Only 9 13 controls stx2+, 8 gene. Comparison profiles obtained PFGE XbaI-digested genomic DNA showed a great diversity isolates, 37 39 analyzed. Cluster analysis wide distribution clinical HUS asymptomatic individuals pigs. Analysis correlation exists genotype pathogenic potential. A larger difference in restriction patterns observed serogroups O26 O111. These results indicate several clones circulate Chile suggest pigs are an important reservoir infections EHEC. Guidelines have been proposed better practices slaughter animals Chile.
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