Resveratrol prevents steroid-induced osteonecrosis of the femoral head via miR-146a modulation.

摘要: The purpose of this study was to investigate the possible use resveratrol (Res) reverse abnormal osteogenesis/osteoclastogenesis activity that occurs during femoral head osteonecrosis and explore detailed mechanisms. Application Res bone marrow-derived mesenchymal stem cells in vitro promoted survival, inhibited apoptosis, downregulated expression reactive oxygen species expression. Moreover, application associated with elevated microRNA-146a (miR-146a) expression, osteogenic differentiation, suppressed osteoclastic which were markedly reversed by miR-146a inhibitor. Histopathological observations micro-computed tomography scanning results indicated Res-treated group had lower incidence better microstructure than untreated group. osteoclastogenesis through altering levels sirtuin1 (Sirt1), nuclear transcription factor-κB (NF-κB), receptor activator NF-κB ligand (RANKL). Simultaneously, treatment improved formation increased β-catenin runt-related factor 2 (Runt2) levels, while reducing forkhead box class O (FOXO) family protein levels. our suggest prevents steroid-induced upregulating miR-146a, thereby stabilizes homeostasis via Wnt/FOXO Sirt1/NF-κB pathways.