Enhanced Antigen Processing of Flagellin Fusion Proteins Promotes the Antigen-Specific CD8+ T Cell Response Independently of TLR5 and MyD88

摘要: Flagellin is a highly effective adjuvant for CD4(+) T cell and humoral immune responses. However, there conflicting data in the literature regarding ability of flagellin to promote CD8(+) response. In this article, we report that immunization wild-type, TLR5(-/-), MyD88(-/-) adoptive transfer recipient mice revealed fusion proteins OVA-specific proliferation independent TLR5 or MyD88 expression by animal. Wild-type TLR5(-/-) APCs were able stimulate high levels vitro response protein containing full-length OVA SIINFEKL epitope 10 flanking amino acids (OVAe), but not added as separate proteins. This effect was conserved regions occurred OVAe alone. Comparison IFN-γ production effector cells higher peptide-MHC I complexes on surface had been pulsed with OVAe-flagellin than OVA. Inhibition proteasome significantly reduced Ag-specific summary, our are consistent conclusion flagellin-OVA induce an epitope-specific facilitating Ag processing through stimulatory signaling via MyD88. Our findings raise possibility might be efficient carrier Ags poorly processed their native state.