microRNA-17 Is the Most Up-Regulated Member of the miR-17-92 Cluster during Early Colon Cancer Evolution.

摘要: Deregulated microRNAs play a role in the development and progression of colon cancer, but little is known about their tissue cell distribution continuum normal mucosa through premalignant adenoma to invasive adenocarcinoma. The aim this study was examine expression pattern miR-17-92 cluster (miR-17, miR-18, miR-19, miR-20 miR-92) as well miR-21, miR-31, miR-135b, miR-145 early clinically diagnosed cancer. MicroRNAs were analysed by chromogenic situ hybridisation normal-adenoma-adenocarcinoma sequence nine adenocarcinomas developed mucosal polyps. Subsequently, selected validated 24 cancer Expression miR-17 confined epithelial cells, levels increased transitional zone from adenomatous tissue. members, miR-19b, miR-20a, miR-92a, followed same pattern, most predominant. An miR-21 found tumour-associated stroma with dramatic increase adenocarcinoma, while number positive fibroblast-like cells lamina propria (stroma) decreased stepwise manner throughout sequence. It concluded that miR-17, changes at stages Thus, these may