Human neural crest-derived postnatal cells exhibit remarkable embryonic attributes either in vitro or in vivo

摘要: During human embryonic development, odontogenic tissues, deriving from the neural crest, remain undifferentiated until adult age. This study was aimed at characterising cells of follicle enveloping dental germ, due to its direct origin crests. Sixty follicles were collected patients aged 18 45 years. research has clarified that follicles, if extracted in a very early stage, when roots did not start be formed, contain lineage cells, characterised by high degree plasticity comparison with other stem cell populations. In particular, we found these share following features ES: (i) levels markers (CD90, TRA1-60, TRA1-81, OCT-4, CD133, and SSEA-4); (ii) mRNA transcripts for Nanog Rex-1; (iii) broader potency, being able differentiate types all three germ layer, including smooth skeletal muscle, osteoblasts, neurons, glial adipocytes; (iv) telomerase activity; (v) ability form embryoid bodies; (vi) ability, after injection murine blastocysts, localised within inner mass; (vii) no teratoma formation injection; (viii) vivo tissue transplantation. Our results demonstrate represent easy accessible extraordinary source pluripotent point out fact they own cardinal feature cells.