Localization of two epitopes of protein L7/L12 to both the body and stalk of the large ribosomal subunit. Immune electron microscopy using monoclonal antibodies.
作者： H M Olson , A Sommer , D S Tewari , R R Traut , D G Glitz
DOI: 10.1016/S0021-9258(19)62705-5
关键词: Protein subunit 、 Immunoelectron microscopy 、 Molecular biology 、 Epitope 、 Large ribosomal subunit 、 Biology 、 Ribosome 、 Ribosomal RNA 、 Ribosomal protein 、 Monoclonal antibody 、 Cell biology 、 Biochemistry
摘要: Four molecules of ribosomal protein L7/L12 are found as two dimers on the Escherichia coli 50 S subunit. Immune electron microscopy using monoclonal antibodies directed against epitopes has allowed placement elements each dimer. One antibody, a determinant in COOH-terminal domain, allows localization identical determinants at or near end subunit stalk. The same antibody was used to place additional periphery stalkless subunits, an area from which stalk might be expected project. A second epitope amino-terminal portion L7/L12, caused loss stalks subunits. micrographs showed symmetrical oligometric complexes dissociated dimeric with bivalent antibody. Antibodies were also seen bind body region sites. results explained by model one dimer exists folded conformation and occurs extended
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sci-hub.st 参考文章(32)
P. Traub, S. Mizushima, C.V. Lowry, M. Nomura, [41] Reconstitution of ribosomes from subribosomal components Methods in Enzymology. ,vol. 20, pp. 391- 407 ,(1971) , 10.1016/S0076-6879(71)20043-4
A Sommer, J R Etchison, G Gavino, N Zecherle, C Casiano, R R Traut, Preparation and characterization of two monoclonal antibodies against different epitopes in Escherichia coli ribosomal protein L7/L12. Journal of Biological Chemistry. ,vol. 260, pp. 6522- 6527 ,(1985) , 10.1016/S0021-9258(18)88812-3
C.C. Lee, B.D. Wells, R.H. Fairclough, C.R. Cantor, Fluorescence studies of the location of proteins L7 and L12 on the Escherichia coli ribosome. Journal of Biological Chemistry. ,vol. 256, pp. 49- 53 ,(1981) , 10.1016/S0021-9258(19)70095-7
D S Tewari, A Sommer, R R Traut, The selective release of one of the two L7/L12 dimers from the Escherichia coli ribosome induced by a monoclonal antibody to the NH2-terminal region. Journal of Biological Chemistry. ,vol. 261, pp. 6919- 6923 ,(1986) , 10.1016/S0021-9258(19)62704-3
W. Möller, P.I. Schrier, J.A. Maassen, A. Zantema, E. Schop, H. Reinalda, A.F.M. Cremers, J.E. Mellema, Ribosomal proteins of Escherichia coli localization and possible molecular mechanism in translation Journal of Molecular Biology. ,vol. 163, pp. 553- 573 ,(1983) , 10.1016/0022-2836(83)90112-2
Ernest Hamel, Mohan Koka, Tokumasa Nakamoto, Requirement of an Escherichia coli 50 S ribosomal protein component for effective interaction of the ribosome with T and G factors and with guanosine triphosphate. Journal of Biological Chemistry. ,vol. 247, pp. 805- 814 ,(1972) , 10.1016/S0021-9258(19)45679-2
R R Traut, J M Lambert, J W Kenny, Ribosomal protein L7/L12 cross-links to proteins in separate regions of the 50 S ribosomal subunit of Escherichia coli. Journal of Biological Chemistry. ,vol. 258, pp. 14592- 14598 ,(1983) , 10.1016/S0021-9258(17)43904-4
H M Olson, P G Grant, B S Cooperman, D G Glitz, Immunoelectron microscopic localization of puromycin binding on the large subunit of the Escherichia coli ribosome. Journal of Biological Chemistry. ,vol. 257, pp. 2649- 2656 ,(1982) , 10.1016/S0021-9258(18)34973-1
C A Cowgill, B G Nichols, J W Kenny, P Butler, E M Bradbury, R R Traut, Mobile domains in ribosomes revealed by proton nuclear magnetic resonance. Journal of Biological Chemistry. ,vol. 259, pp. 15257- 15263 ,(1984) , 10.1016/S0021-9258(17)42543-9
Hiroaki Tokimatsu, William A. strycharz, Albert E. Dahlberg, Gel electrophoretic studies on ribosomal proteins L7L12 and the Escherichia coli 50 s subunit Journal of Molecular Biology. ,vol. 152, pp. 397- 412 ,(1981) , 10.1016/0022-2836(81)90250-3