Interruption of reverse transcriptase inhibitors or a switch from reverse transcriptase to protease inhibitors resulted in a fast reappearance of virus strains with a reverse transcriptase inhibitor-sensitive genotype.

摘要: Objectives: To study the effect of interruption reverse transriptase inhibitor (RTI) therapy or a switch from RTI to protease inhibitors, on genotypic drug-resistance pattern plasma HIV-1. Methods: Nine patients who completely stopped all medication, and five switched treatment with regimen containing inhibitors only, were studied. Direct sequencing HIV-1 RT gene was performed follow-up samples taken before after treatment. Results: Comparison amino acid sequence genes in successive showed rapid reappearance wild-type viral variants 12 14 Wild-type virus replaced mutant strains days 2 months therapy, even long history. Conclusion: The results this indicate sustained lower fitness vivo. As result, remains capable outcompeting very fast removal drug. These findings highlight importance 'treatment history' addition phenotypic markers determined at one time-point, when making therapeutic decisions for patients.