Recruitment of conjugative DNA transfer substrate to Agrobacterium type IV secretion apparatus.

摘要: Bacterial type IV secretion system (T4SS) belongs to a growing class of evolutionarily conserved transporters that translocate DNA and proteins into wide variety organisms including bacterial eukaryotic cells. Archetypal is the Agrobacterium tumefaciens VirB/D4 T4SS transfers oncogenic T-DNA various cells, which transferred as nucleoprotein T-complex with VirD2 pilot protein. As derivative plasmid conjugation systems, can also transfer certain mobilizable plasmids like VirE2 VirF, although it unknown how membrane-bound recruits different substrates. Here, we show cytoplasmic VirD2-binding protein (VBP) involved in recruitment energizing components T4SS, VirD4, VirB4, VirB11. VBP important for conjugative independent VirB/D4. These data indicate functions previously unrecognized recruiting helps couple substrates appropriate transport sites transfers. has three functionally redundant homologs, similar homologs be found genomes, suggesting uncharacterized