Transformation of a rat cell line by an adenovirus type 12 DNA fragment
作者： Shoki Yang , Setsuko Ojima , Kei Fujinaga , Kazuko Shiroki , Hiroto Shimojo
DOI: 10.1016/0042-6822(77)90044-7
关键词: Gene 、 DNA 、 Biology 、 Antigen 、 Genome 、 Transformation (genetics) 、 Cell culture 、 In vitro recombination 、 Molecular biology 、 Nucleotide 、 Virology
摘要: Abstract A clonal rat embryo cell line, 3Y1, was transformed by the Hind III-I·J fragment of adenovirus 7 DNA (left 8.1% whole Adz DNA), one partial digestion products with Hin dIII, and Ad7 DNA. Most, if not all, nucleotide sequences dIII-I·J were present in cells fragment, whereas approximately left two thirds viral genome These results indicate that transforming gene is located mapped at 8% end genome.
elsevier.com
sci-hub.se 参考文章(28)
Maurice Green, Magdalena Piña, Biochemical studies on adenovirus multiplication: IV. Isolation, purification, and chemical analysis of adenovirus☆ Virology. ,vol. 20, pp. 199- 207 ,(1963) , 10.1016/0042-6822(63)90157-0
Hamilton O. Smith, K.W. Welcox, A Restriction enzyme from Hemophilus influenzae: I. Purification and general properties Journal of Molecular Biology. ,vol. 51, pp. 379- 391 ,(1970) , 10.1016/0022-2836(70)90149-X
S.J. Flint, Joe Sambrook, J.F. Williams, Phillip A. Sharp, Viral nucleic acid sequences in transformed cells: IV. A study of the sequences of adenovirus 5 DNA and RNA in four lines of Adenovirus 5-transformed rodent cells using specific fragments of the viral genome Virology. ,vol. 72, pp. 456- 470 ,(1976) , 10.1016/0042-6822(76)90174-4
J Ortin, K H Scheidtmann, R Greenberg, M Westphal, W Doerfler, Transcription of the genome of adenovirus type 12. III. Maps of stable RNA from productively infected human cells and abortively infected and transformed hamster cells. Journal of Virology. ,vol. 20, pp. 355- 372 ,(1976) , 10.1128/JVI.20.2.355-372.1976
Carel Mulder, Phillip A. Sharp, Hajo Delius, Ulf Pettersson, Specific Fragmentation of DNA of Adenovirus Serotypes 3, 5, 7, and 12, and Adeno-Simian Virus 40 Hybrid Virus Ad2+ND1 by Restriction Endonuclease R· EcoRI Journal of Virology. ,vol. 14, pp. 68- 77 ,(1974) , 10.1128/JVI.14.1.68-77.1974
K Fujinaga, K Sekikawa, H Yamazaki, Method for determination of nucleotide sequence homology between viral genomes by DNA reassociation kinetics. Journal of Virology. ,vol. 15, pp. 466- 470 ,(1975) , 10.1128/JVI.15.3.466-470.1975
F.L. Graham, A.J. van der Eb, A new technique for the assay of infectivity of human adenovirus 5 DNA Virology. ,vol. 52, pp. 456- 467 ,(1973) , 10.1016/0042-6822(73)90341-3
M. Green, M. Pina, BIOCHEMICAL STUDIES ON ADENOVIRUS MULTIPLICATION, VI. PROPERTIES OF HIGHLY PURIFIED TUMORIGENIC HUMAN ADENOVIRUSES AND THEIR DNA's Proceedings of the National Academy of Sciences of the United States of America. ,vol. 51, pp. 1251- 1259 ,(1964) , 10.1073/PNAS.51.6.1251
Hiroto Shimojo, Hiroshi Yamamoto, Chikako Abe, Differentiation of adenovirus 12 antigens in cultured cells with immunofluorescent analysis Virology. ,vol. 31, pp. 748- 752 ,(1967) , 10.1016/0042-6822(67)90213-9
Lawrence D. Gelb, David E. Kohne, Malcolm A. Martin, Quantitation of Simian virus 40 sequences in African green monkey, mouse and virus-transformed cell genomes. Journal of Molecular Biology. ,vol. 57, pp. 129- 145 ,(1971) , 10.1016/0022-2836(71)90123-9