Patterns of starchy endosperm acidification and protease gene expression in wheat grains following germination
作者： Fernando Domı́nguez , Francisco J. Cejudo
DOI: 10.1104/PP.119.1.81
关键词: Gibberellic acid 、 Aleurone 、 Biochemistry 、 Gene expression 、 Incubation 、 Carboxypeptidase 、 Endosperm 、 Gibberellin 、 Imbibition 、 Biology
摘要: Cereal aleurone responses to gibberellic acid (GA3) include activation of synthesis hydrolytic enzymes and acidification the external medium. We have studied effect pH incubation medium on response wheat (Triticum aestivum) cells GA3. De-embryonated half grains show capacity for GA3-activated when is carried out at 6.0 7.0 but not lower pHs. In addition, activating GA3 expression carboxypeptidase III thiol protease genes more efficient hormone treatment neutral pH. situ staining showed that starchy endosperm takes place upon imbibition advances from embryo distal part grain. hybridization experiments a similar pattern gene, which up-regulated by in cells. However, gene precedes acidification. These findings imply vivo GA perception layer suggest regulated germinated grains.
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oup.com参考文章(29)
Fernando Dominguez, Francisco J. Cejudo, Pattern of endoproteolysis following wheat grain germination Physiologia Plantarum. ,vol. 95, pp. 253- 259 ,(1995) , 10.1111/J.1399-3054.1995.TB00835.X
Alberto Hamabata, Mitla García-Maya, Teresa Romero, Irma Bernal-Lugo, Kinetics of the Acidification Capacity of Aleurone Layer and Its Effect upon Solubilization of Reserve Substances from Starchy Endosperm of Wheat Plant Physiology. ,vol. 86, pp. 643- 644 ,(1988) , 10.1104/PP.86.3.643
Richard Hooley, MichaelH. Beale, SallyJ. Smith, Gibberellin perception at the plasma membrane of Avena fatua aleurone protoplasts. Planta. ,vol. 183, pp. 274- 280 ,(1991) , 10.1007/BF00197799
Karin M.C. Sinjorgo, Mariska A. de Vries, Jolanda C. Heistek, Mieke J. van Zeijl, Simone W. van der Veen, Anneke C. Douma, The effect of external ph on the gibberellic acid response of barley aleurone Journal of Plant Physiology. ,vol. 142, pp. 506- 509 ,(1993) , 10.1016/S0176-1617(11)81260-5
FJ Cejudo, G Murphy, C Chinoy, DC Baulcombe, A gibberellin-regulated gene from wheat with sequence homology to cathepsin B of mammalian cells. Plant Journal. ,vol. 2, pp. 937- 948 ,(1992) , 10.1046/J.1365-313X.1992.T01-7-00999.X
David C. Baulcombe, Dominique Buffard, Gibberellic-acid-regulated expression of α-amylase and six other genes in wheat aleurone layers Planta. ,vol. 157, pp. 493- 501 ,(1983) , 10.1007/BF00396879
Marı́a-Cruz González, Lidia Osuna, Cristina Echevarrı́a, Jean Vidal, Francisco J. Cejudo, Expression and Localization of Phosphoenolpyruvate Carboxylase in Developing and Germinating Wheat Grains Plant Physiology. ,vol. 116, pp. 1249- 1258 ,(1998) , 10.1104/PP.116.4.1249
Peter K. Macnicol, John V. Jacobsen, Endosperm Acidification and Related Metabolic Changes in the Developing Barley Grain Plant Physiology. ,vol. 98, pp. 1098- 1104 ,(1992) , 10.1104/PP.98.3.1098
Marjatta Salmenkallio, Tuomas Sopanen, Amino Acid and Peptide Uptake in the Scutella of Germinating Grains of Barley, Wheat, Rice, and Maize Plant Physiology. ,vol. 89, pp. 1285- 1291 ,(1989) , 10.1104/PP.89.4.1285
Richard Hooley, GIBBERELLINS : PERCEPTION, TRANSDUCTION AND RESPONSES Plant Molecular Biology. ,vol. 26, pp. 1529- 1555 ,(1994) , 10.1007/BF00016489