Nutrition during mid to late pregnancy does not affect the birthweight response to mid pregnancy shearing
作者： P. R. Kenyon , S. T. Morris , D. K. Revell , S. N. McCutcheon
DOI: 10.1071/AR01001
关键词: Sperm 、 Andrology 、 Pregnancy 、 Biotechnology 、 Affinity chromatography 、 Molecular mass 、 Biology 、 Size-exclusion chromatography 、 Sephadex 、 Concanavalin A 、 Gestation
摘要: This study was designed to determine whether dam nutrition during mid latepregnancy influences birthweight responses from pregnancy shearing.Romney-cross ewes were either shorn pregnancy(n = 68) or left unshorn (n= 66). Ewes offered a maintenance level of feed (a ofnutrition that enabled the maintain conceptus-free liveweight) aed apparenthomogeneity by using various biochemical fractionation procedures, such assolubilization with Triton X-100, sephadex gel filtration chromatography,concanavalin A–sepharose affinity chromatography anddiethylaminoethyl–cellulose ion-exchange chromatography. The isolatedPPase has molecular mass approximately 36 kDa and an isoelectric point of5.95. Sperm surface topography enzyme investigated usingfluorescein isothiocyanate-conjugated antibody purified PPase. Theimmunofluorescent studies have demonstrated isolated PPase islocalized on external viable sperm. Immunocytochemical studiesalso revealed marked topographical alteration ecto-PPase duringepididymal transit male gametes. Immunoreactivity observed all overthe caput sperm, but restricted primarily anterior tipof head in corpus sperm posterior part caudasperm cells. maturation-dependent decrease activity alsoconfi
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