Characterization of the lymphoid stroma in Warthin's tumor of salivary gland by immunohistochemistry, heavy chain gene and Bcl-2 gene rearrangement.

摘要: Warthin's tumor is rarely associated with malignant lymphoma. Only 18 cases were reported in the literature so far. In most latter a low grade process, including Marginal zone/Mucosa lymphoid tissue (MALT) type lymphoma, follicular and diffuse large cell lymphoma which may arise de novo or secondary to This study was conducted determine prevalence of occult B monoclones genetic alterations tumor. Fourteen stained antibodies CD3, CD20, kappa lambda light chains. On six randomly selected tumor, polymerase chain reaction (PCR) IgH gene rearrangement (IgH-GR) performed on genomic DNA extracted from formalin-fixed paraffin embedded tissue. One case primary salivary gland indolent B-cell 3 sialadenitis analyzed by same methods for comparison. all stroma phenotype concentrated germinal centers. T cells mostly located between No restriction demonstrable immunostains. Molecular studies failed show IgH-GR FISH showed polyclonal PCR. contrast, proliferation small minor component reactive cells. bcl-2 translocation monoclonality Our concludes that reactive.