Detection ofFusarium trichothecenes (nivalenol, deoxynivalenol, fusarenone and 3-acetyldeoxynivalenol) by high-performance liquid chromatography

作者: A. Visconti , A. Bottalico

DOI: 10.1007/BF02259348

关键词: Uv detectionHigh-performance liquid chromatographyDetection limitChemistryChromatography

摘要: Separation and determination ofFusarium trichothecenes: nivalenol, deoxynivalenol, fusarenone 3-acetyldeoxynivalenol, were carried out by highperformance liquid chromatography with UV detection, methanol—water (30/70) as mobile-phase on Lichrosorb RP-18 column. Detection limits 2, 3, 3 5ng per injection of respectively. The method appears to be adequate for detection the above trichothecenes in contaminated corn rice well cultures spp. A preliminary TLC purification was necessary corn.

参考文章(10)
Rainer Schmidt, Elena Ziegenhagen, Klaus Dose, High-performance liquid chromatography of trichothecenes : I. Detection of T-2 toxin and HT-2 toxin Journal of Chromatography A. ,vol. 212, pp. 370- 373 ,(1981) , 10.1016/S0021-9673(01)84052-8
R. M. Eppley, Trichothecenes and their analysis Journal of the American Oil Chemists' Society. ,vol. 56, pp. 824- 829 ,(1979) , 10.1007/BF02909527
Cs. Szathmáry, J. Galácz, L. Vida, G. Alexander, Capillary gas chromatographic-mass spectrometric determination of some mycotoxins causing fusariotoxicoses in animals Journal of Chromatography A. ,vol. 191, pp. 327- 331 ,(1980) , 10.1016/S0021-9673(00)86397-9
H. Schweighardt, J. Böhm, A. M. Abdelhamid, J. Leibetseder, M. Schuh, E. Glawischnig, Analysis of the fusariotoxins zearalenone and vomitoxin (deoxynivalenol) in human foods and animal feeds by high-performance liquid chromatography (HPLC) Chromatographia. ,vol. 13, pp. 447- 450 ,(1980) , 10.1007/BF02261528
R. J. Maxwell, K. A. Reimann, K. Percell, Determination of the Unsaponifiable Matter in Fatty Acids by a Rapid Column Method Journal of the American Oil Chemists' Society. ,vol. 58, pp. 1002- 1004 ,(1981) , 10.1007/BF02659783
F. Palmisano, A. Visconti, A. Bottalico, P. Lerario, P. G. Zambonin, Differential-pulse polarography of trichothecene toxins: detection of deoxynivalenol in corn The Analyst. ,vol. 106, pp. 992- 998 ,(1981) , 10.1039/AN9810600992
G. A. Bennett, R. E. Peterson, R. D. Plattner, O. L. Shotwell, Isolation and purification of deoxynivalenol and a new trichothecene by high pressure liquid chromatography Journal of the American Oil Chemists' Society. ,vol. 58, ,(1981) , 10.1007/BF02679312
S. V. Pathre, C. J. Mirocha, Trichothecenes: Natural occurrence and potential hazard Journal of the American Oil Chemists' Society. ,vol. 56, pp. 820- 823 ,(1979) , 10.1007/BF02909526
M. Jemmali, Y. Ueno, K. Ishii, C. Frayssinet, M. Etienne, Natural occurrence of trichothecenes (nivalenol, deoxynivalenol, T2) and zearalenone in corn. Cellular and Molecular Life Sciences. ,vol. 34, pp. 1333- 1334 ,(1978) , 10.1007/BF01981454