High level Escherichia coli expression and production of a bivalent humanized antibody fragment.
作者： Paul Carter , Robert F. Kelley , Maria L. Rodrigues , Brad Snedecor , Michael Covarrubias
DOI: 10.1038/NBT0292-163
关键词: Humanized antibody 、 Antibody 、 Bivalent (genetics) 、 Escherichia coli 、 Periplasmic space 、 Cysteine 、 Molecular biology 、 Biology 、 Antigen 、 Enterobacteriaceae
摘要: … Surprisingly, this high expression level of Fab′ in the … ′ with the single hinge cysteine in the free thiol state, allowing F(ab′) 2 … coli expression of bivalent antibody fragments for human …
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