Spatial patterning of endothelial cells and vascular network formation using ultrasound standing wave fields

摘要: The spatial organization of cells is essential for proper tissue assembly and organ function. Thus, successful engineering complex tissues organs requires methods to control cell in three dimensions. In particular, technologies that facilitate endothelial alignment vascular network formation three-dimensional constructs would provide a means supply oxygen nutrients newly forming tissue. Acoustic radiation forces associated with ultrasound standing wave fields can rapidly non-invasively organize into distinct multicellular planar bands within collagen gels. Results presented herein demonstrate the pattern gels be controlled by design acoustic parameters sound field. Different field exposure were used either loosely aggregated or densely packed bands. rate vessel morphology resulting networks affected initial density ultrasound-induced cells. Ultrasound rapid, non-invasive approach three-dimensions direct engineered constructs.