Magnetically-assisted surface enhanced raman spectroscopy (MA-SERS) for label-free determination of human immunoglobulin G (IgG) in blood using Fe3O4@Ag nanocomposite.

作者: Anna Balzerova , Ariana Fargasova , Zdenka Markova , Vaclav Ranc , Radek Zboril

DOI: 10.1021/AC503347H

关键词: Complex matrixNanotechnologySpectrum analysisSilver nanoparticleLabel freeNanocompositeHuman bloodSurface-enhanced Raman spectroscopyChemistryHuman Immunoglobulin G

摘要: Development of methods allowing determination even ultralow levels immunoglobulins in various clinical samples including whole human blood and plasma is a particular scientific challenge, especially due to many essential discoveries the fields immunology medicine past few decades. The IgG usually performed using an enzymatic approach, followed by colorimetric or fluorimetric detection. However, limitations these relate their complicated setup stringent requirements concerning sample purity. Here, we present novel approach based on magnetically assisted surface enhanced Raman spectroscopy (MA/SERS), which utilizes Fe3O4@Ag@streptavidin@anti-IgG nanocomposite with strong magnetic properties efficient SERS enhancement factor conferred Fe3O4 particles silver nanoparticles, respectively. Such offers possibility separating target efficiently from complex matrix simple application external m...

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