作者: Anna Balzerova , Ariana Fargasova , Zdenka Markova , Vaclav Ranc , Radek Zboril
DOI: 10.1021/AC503347H
关键词: Complex matrix 、 Nanotechnology 、 Spectrum analysis 、 Silver nanoparticle 、 Label free 、 Nanocomposite 、 Human blood 、 Surface-enhanced Raman spectroscopy 、 Chemistry 、 Human Immunoglobulin G
摘要: Development of methods allowing determination even ultralow levels immunoglobulins in various clinical samples including whole human blood and plasma is a particular scientific challenge, especially due to many essential discoveries the fields immunology medicine past few decades. The IgG usually performed using an enzymatic approach, followed by colorimetric or fluorimetric detection. However, limitations these relate their complicated setup stringent requirements concerning sample purity. Here, we present novel approach based on magnetically assisted surface enhanced Raman spectroscopy (MA/SERS), which utilizes Fe3O4@Ag@streptavidin@anti-IgG nanocomposite with strong magnetic properties efficient SERS enhancement factor conferred Fe3O4 particles silver nanoparticles, respectively. Such offers possibility separating target efficiently from complex matrix simple application external m...