Shiga Toxin-Producing Escherichia Coli

摘要: This chapter discusses the emergence and impact of shiga toxin-producing Escherichia coli (STEC) in human disease, biology Shiga toxins (Stx) family, approaches to diagnosis, treatment, prevention infection with STEC. The occurrence seminal STEC outbreaks highlighted need implement laboratory methods readily detect E. O157:H7. Although early characterization strains was made possible by Whittam’s multilocus enzyme electrophoresis (MLEE) method, genetic techniques now allow comparison at nucleotide level. current model predominant pathway which Stx intoxicates sensitive cells is as follows: (i) B pentamer holotoxin binds Gb3 within lipid rafts; (ii) entire receptor-holotoxin complex endocytosed; (iii) moves retrograde transport Golgi then endoplasmic reticulum; (iv) A1 subunit released into cytoplasm, where it targets ribosome. transduction stx genes via bacteriophages crucial STEC, these toxin-converting phages also contributes significantly degree toxin expression hence virulence exhibited pathogenicity various that synthesize different types Stx2 cannot be compared directly because are not isogenic, authors have found an O91 strain produces virulent streptomycin-treated mouse for infection, whereas produce Stx2d-activatable highly those mice.