Gene targeting with retroviral vectors: recombination by gene conversion into regions of nonhomology.
作者： J Ellis , A Bernstein
DOI: 10.1128/MCB.9.4.1621
关键词: Mutagenesis (molecular biology technique) 、 Homologous recombination 、 Biology 、 Mutant 、 Transfection 、 Genetics 、 Genetic recombination 、 Gene targeting 、 Gene conversion 、 Molecular biology 、 Gene
摘要: We have designed and constructed integration-defective retroviral vectors to explore their potential for gene targeting in mammalian cells. Two nonoverlapping deletion mutants of the bacterial neomycin resistance (neo) were used detect homologous recombination events between viral chromosomal sequences. Stable neo correction selected at a frequency approximately 1 G418r cell per 3 x 10(6) infected Analysis functional independent targeted clones indicated that unintegrated linear DNA recombined with target by conversion variable distances into regions nonhomology. In addition, transient which associated complete loss sequences observed. These results demonstrated can recombine rodent human
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