作者: Mutsuo Yamada , Katsuhiko Suzuki , Satoru Kudo , Manabu Totsuka , Tadashi Simoyama
DOI: 10.1002/(SICI)1522-7243(200001/02)15:1<15::AID-BIO570>3.0.CO;2-O
关键词: Zymosan 、 Myeloperoxidase 、 Phorbol Myristate Acetate 、 Stimulation 、 Endocrinology 、 Sodium azide 、 Chemistry 、 Internal medicine 、 Lucigenin 、 Catalase 、 Reactive oxygen species 、 Immunology 、 Biophysics 、 Chemistry (miscellaneous)
摘要: In order to investigate the effect of exercise on capacity neutrophils produce reactive oxygen species (ROS), eight male cross-country skiers underwent maximal exercise. Peripheral blood samples were taken pre-exercise, 0 h, 1 h, and 2 h after finishing Leukocyte counts significantly increased (p < 0.05), particularly lymphocytes just period (0 h) again compared with pre-exercise values. The isolated ROS was assessed by lucigenin (Lg)-dependent chemiluminescence (CL) luminol (Lm)-dependent CL stimulation opsonized zymosan (OZ) phorbol myristate acetate (PMA). Just exercise, LgCL response not affected, while LmCL mixed sodium azide, which inhibits catalase myeloperoxidase (MPO) activity, enhanced (p < 0.05). addition, level serum growth hormone cortisol also 1 h These data indicated that only mobilized from marginated pools into circulation, but caused generation. Copyright © 2000 John Wiley & Sons, Ltd.