Effect of exhaustive exercise on human neutrophils in athletes

作者: Mutsuo Yamada , Katsuhiko Suzuki , Satoru Kudo , Manabu Totsuka , Tadashi Simoyama

DOI: 10.1002/(SICI)1522-7243(200001/02)15:1<15::AID-BIO570>3.0.CO;2-O

关键词: ZymosanMyeloperoxidasePhorbol Myristate AcetateStimulationEndocrinologySodium azideChemistryInternal medicineLucigeninCatalaseReactive oxygen speciesImmunologyBiophysicsChemistry (miscellaneous)

摘要: In order to investigate the effect of exercise on capacity neutrophils produce reactive oxygen species (ROS), eight male cross-country skiers underwent maximal exercise. Peripheral blood samples were taken pre-exercise, 0 h, 1 h, and 2 h after finishing Leukocyte counts significantly increased (p < 0.05), particularly lymphocytes just period (0 h) again compared with pre-exercise values. The isolated ROS was assessed by lucigenin (Lg)-dependent chemiluminescence (CL) luminol (Lm)-dependent CL stimulation opsonized zymosan (OZ) phorbol myristate acetate (PMA). Just exercise, LgCL response not affected, while LmCL mixed sodium azide, which inhibits catalase myeloperoxidase (MPO) activity, enhanced (p < 0.05). addition, level serum growth hormone cortisol also 1 h These data indicated that only mobilized from marginated pools into circulation, but caused generation. Copyright © 2000 John Wiley & Sons, Ltd.

参考文章(31)
K. Kokot, R. M. Schaefer, M. Teschner, U. Gilge, R. Plass, A. Heidland, Activation of leukocytes during prolonged physical exercise. Advances in Experimental Medicine and Biology. ,vol. 240, pp. 57- 63 ,(1988) , 10.1007/978-1-4613-1057-0_6
Murray A. Mittleman, Malcolm Maclure, Geoffrey H. Tofler, Jane B. Sherwood, Robert J. Goldberg, James E. Muller, Triggering of Acute Myocardial Infarction by Heavy Physical Exertion -- Protection against Triggering by Regular Exertion New England Journal of Medicine. ,vol. 329, pp. 1677- 1683 ,(1993) , 10.1056/NEJM199312023292301
M C Seeds, D A Bass, P Szejda, C E McCall, P Olbrantz, Subpopulations of neutrophils with increased oxidative product formation in blood of patients with infection. Journal of Immunology. ,vol. 136, pp. 860- 866 ,(1986)
N C C Sharp, Y Koutedakis, Sport and the overtraining syndrome: Immunological aspects British Medical Bulletin. ,vol. 48, pp. 518- 533 ,(1992) , 10.1093/OXFORDJOURNALS.BMB.A072560
B. J. Freund, E. M. Shizuru, G. M. Hashiro, J. R. Claybaugh, Hormonal, electrolyte, and renal responses to exercise are intensity dependent. Journal of Applied Physiology. ,vol. 70, pp. 900- 906 ,(1991) , 10.1152/JAPPL.1991.70.2.900
K. Suzuki, S. Naganuma, M. Totsuka, K.-J. Suzuki, M. Mochizuki, M. Shiraishi, S. Nakaji, K. Sugawara, Effects of Exhaustive Endurance Exercise and its One-Week Daily Repetition on Neutrophil Count and Functional Status in Untrained Men International Journal of Sports Medicine. ,vol. 17, pp. 205- 212 ,(1996) , 10.1055/S-2007-972833
Holger Gabriel, Lothar Schwarz, Petra Born, Wilfried Kindermann, Differential mobilization of leucocyte and lymphocyte subpopulations into the circulation during endurance exercise. European Journal of Applied Physiology. ,vol. 65, pp. 529- 534 ,(1992) , 10.1007/BF00602360
JOHN MIHRAN DAVIS, JAMES D. ALBERT, KEVIN J. TRACY, STEVE E. CALVANO, STEPHEN F. LOWRY, G. T. SHIRES, ROGER W. YURT, Increased Neutrophil Mobilization and Decreased Chemotaxis During Cortisol and Epinephrine Infusions Journal of Trauma-injury Infection and Critical Care. ,vol. 31, pp. 725- 732 ,(1991) , 10.1097/00005373-199106000-00001
Takashi Kikuchi, Katsuhiko Suzuki, Tatsuya Abe, Hideki Satoh, Tetsu Endoh, Hiroko Hasegawa, Shigeyuki Nakaji, Kazuo Sugawara, Takashi Kumae, Measurement of chemiluminescence from neutrophils in a 96-well microplate using Lumi Box U-800 II Journal of Bioluminescence and Chemiluminescence. ,vol. 12, pp. 149- 153 ,(1997) , 10.1002/(SICI)1099-1271(199705/06)12:3<149::AID-BIO440>3.0.CO;2-O
D.A. McCarthy, M.M. Dale, The leucocytosis of exercise. A review and model. Sports Medicine. ,vol. 6, pp. 333- 363 ,(1988) , 10.2165/00007256-198806060-00002