Narrowing down the apricot Plum pox virus resistance locus and comparative analysis with the peach genome syntenic region.

摘要: SUMMARY Sharka disease, caused by the Plum pox virus (PPV), is one of main limiting factors for stone fruit crops worldwide. Only a few resistance sources have been found in apricot (Prunus armeniaca L.), and most studies located major PPV locus (PPVres) on linkage group 1 (LG1). However, mapping accuracy was not sufficiently reliable PPVres predicted within low confidence interval. In this study, we constructed two high-density simple sequence repeat (SSR) improved maps with 0.70 0.68 markers/cm, corresponding to LG1 ‘Lito’ ‘Goldrich’ PPV-resistant cultivars, respectively. Using these maps, excluding genotype–phenotype incongruent individuals, new binary trait (BTL) analysis performed, narrowing down support intervals 7.3 5.9 cm ‘Goldrich’, Subsequently, 71 overlapping oligonucleotides (overgo) probes were hybridized against an bacterial artificial chromosome (BAC) library, identifying 870 single BACs from which 340 anchored onto map region approximately 30–40 cm encompassing PPVres. Partial BAC contigs assigned allelic haplotypes (resistant/susceptible) built high-information content fingerprinting (HICF). addition, total 300 BAC-derived sequences obtained, 257 showed significant homology peach genome scaffold_1 LG1. According syntenic sequence, 2.16 Mb candidate genes identified.