Photobleaching GFP reveals protein dynamics inside live cells
作者： Jamie White , Ernst Stelzer
DOI: 10.1016/S0962-8924(98)01433-0
关键词: Green fluorescent protein 、 Gfp fusion 、 Fluorescence recovery after photobleaching 、 Cell biology 、 Fusion protein 、 Biology 、 Photobleaching 、 Biophysics 、 Protein dynamics
摘要: Cell biologists have used photobleaching to investigate the lateral mobility of fluorophores on cell surface since 1970s. Fusions green fluorescent protein (GFP) specific proteins extend techniques investigation dynamics within cell, leading renewed interest in experiments. This article revisits general concepts, reviews what can be learned from them and discusses applications illustrating potential GFP fusion inside living cells.
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sci-hub.se 参考文章(24)
John F. Presley, Nelson B. Cole, Trina A. Schroer, Koret Hirschberg, Kristien J. M. Zaal, Jennifer Lippincott-Schwartz, ER-to-Golgi transport visualized in living cells Nature. ,vol. 389, pp. 81- 85 ,(1997) , 10.1038/38001
Leslie A. Leinwand, David L. Spector, Robert D. Goldman, Cells: a laboratory manual Cold Spring Harbor Laboratory Press. pp. 1- ,(1997)
Paul C. Goodwin, GFP biofluorescence: imaging gene expression and protein dynamics in living cells. Design considerations for a fluorescence imaging laboratory. Methods in Cell Biology. ,vol. 58, pp. 343- 367 ,(1998) , 10.1016/S0091-679X(08)61964-0
Takao Nakata, Sumio Terada, Nobutaka Hirokawa, Visualization of the Dynamics of Synaptic Vesicle and Plasma Membrane Proteins in Living Axons Journal of Cell Biology. ,vol. 140, pp. 659- 674 ,(1998) , 10.1083/JCB.140.3.659
Kala Subramanian, Tobias Meyer, Calcium-Induced Restructuring of Nuclear Envelope and Endoplasmic Reticulum Calcium Stores Cell. ,vol. 89, pp. 963- 971 ,(1997) , 10.1016/S0092-8674(00)80281-0
R. Swaminathan, C.P. Hoang, A.S. Verkman, Photobleaching recovery and anisotropy decay of green fluorescent protein GFP-S65T in solution and cells: cytoplasmic viscosity probed by green fluorescent protein translational and rotational diffusion. Biophysical Journal. ,vol. 72, pp. 1900- 1907 ,(1997) , 10.1016/S0006-3495(97)78835-0
Malea Kneen, Javier Farinas, Yuxin Li, A.S. Verkman, Green fluorescent protein as a noninvasive intracellular pH indicator. Biophysical Journal. ,vol. 74, pp. 1591- 1599 ,(1998) , 10.1016/S0006-3495(98)77870-1
D. Axelrod, D.E. Koppel, J. Schlessinger, E. Elson, W.W. Webb, Mobility measurement by analysis of fluorescence photobleaching recovery kinetics Biophysical Journal. ,vol. 16, pp. 1055- 1069 ,(1976) , 10.1016/S0006-3495(76)85755-4
N. B. Cole, C. L. Smith, N. Sciaky, M. Terasaki, M. Edidin, J. Lippincott-Schwartz, Diffusional mobility of Golgi proteins in membranes of living cells Science. ,vol. 273, pp. 797- 801 ,(1996) , 10.1126/SCIENCE.273.5276.797
Fan Yang, Larry G. Moss, George N. Phillips, The molecular structure of green fluorescent protein Nature Biotechnology. ,vol. 14, pp. 1246- 1251 ,(1996) , 10.1038/NBT1096-1246